Is there a way to quantify a western without using image j or image studio?

07 February 2019 0 1K Report

I lost my flash drive a few weeks back and had some unquantified westerns. Fortunately I saved the images as screenshots onto my computer. However, when I import the images into image studio or image j the screenshotted image is too intense to be quantified. Does anyone know a way around this so that I could still use this data?

Badges
Science topic

More Carly Lancaster's questions See All

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why differences in base pair peak size from 1-step to 2-step metabarcoding (16S rRNA)?

Hi all, We have been trying to figure out why we see such differences in our base pair (bp) fragment size between doing a 1-step PCR reaction vs a 2-step PCR reaction to prepare metabarcoding...

20 December 2023 7,616 0 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,960 1 View

Why am I getting inconsistent results from my ChIP PCR?

One of the times I performed this ChIP I got strong binding at the locus I amplified, but I have not been able to reproduce this result after many many tries. However, I did get the protein I am...

10 December 2020 7,510 1 View

ChIP: why is my cell solution viscous after harvest?

I have performed a ChIP (on 15cm plates) by making a fresh PFA solution, adding it to a 1% final concentration to my cells for 10 minutes and neutralizing the PFA with 2.0M glycine added to a...

21 November 2020 708 2 View

Has anyone used the DSS crosslinker for ChIP?

I am hoping to crosslink the protein-protein interactions in cells as well as the protein-DNA interactions (for which I use formaldehyde). Has anyone tried using the crosslinker DSS for this purpose?

03 August 2020 2,030 2 View

How can I digest cells embedded in a gelatin hydrogel for analysis?

I am 3d printing gelatin hydrogel with fibroblasts embedded and I would like to access how long the fibroblasts remain alive in culture/ how long they retain their functionality. I plan to perform...

17 June 2020 5,022 1 View

Why aren't there spaces between the bands in my protein gel?

I ran a 5-15% gradient gel that I handmade and ran it overnight. I also left an empty lane between each sample and they still managed to spread into one another. I loaded 170 ug of sample (I know...

14 May 2019 8,915 5 View

Protein Lysis buffer for IP and Mass Spect?

I am immunoprecipitating a protein for analysis by mass spect and I was told to minimize tris and also PEG for a clear analysis by mass spectrometry. I lysed in RIPA buffer with 50 mM tris and 1%...

15 April 2019 8,139 0 View

Are there any papers on demand or revenue drivers in B2B sales/booking processes?

A business will not purchase more of a product when its price is lowered. They must first have a need for it. In the industrial setting, There is rarely a hidden market that can be reached by...

20 February 2019 7,638 2 View

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

Do you know best mines of western part of Afghanistan?

I want to know more about Mn deposits in west of Afghanistan.

07 August 2024 3,427 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Hello, regarding Mxene 2D titanium carbide?

I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.

02 August 2024 6,789 4 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View