It would be great to routinely concentrate sperm without the need to centrifuge the stallion semen.
I heard from several people that they rather use centrifugation, because the use of this fiter is rather cumbersome. I am not sure if that is true, but I will give it a try. Thanks.
All filtration methods produce a dilution of spermatozoa. The method of choice arises from your need to concentrate them. If it is to freeze samples or work in vitro. A good working method is the use of Sephadex columns, but always taking into account the final use of the material.
Do you have a protocol for concentrating stallion sperm with (what type of) Sephadex?
As is a washing methods, you will dilute the sample, but get better sperms. So, that is the question, what are you doing with the sample? In vitro work or freezing?
You can simultaneously deplete moribund sperm and enrich and concentrate viable sperm with our magnetic particles. My email is [email protected] Our website is www.clemente-associates.com
Henry
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