Is there an online or standalone tool for analyzing flux balance analysis of E.coli gene knockouts which also considers the regulatory controls ex. RFBA, SRFBA, PROM etc.?
Take a look the a the link below.It may come handy. You must first choose the metabolic network you want for example E.coli in your case. Then you can perform FBAonline, But I think Matlab Cobra toolbox is a better choice although it's not online