Hi, I am studying pretreatment of lignocellulosic materials. During pretreatment, the inhibitors like HMF and furfural are occurred. Therefore, I am required to make their analysis.
Dear Mustafa,
You may use UV-Visible spectroscopy and/or 1H NMR spectroscopy to analyse your samples. The UV-Visible spectrum of furfurals/HMF solution has a distinct
peak at around 284 nm with a corresponding extinction coefficient (e) value of 16400 M-1 cm-1. The yield can be measured calculated from the measured absorbance values at 284 nm and the extinction coefficient value.
For H NMR method, you will need to add a known concentration of internal standard (mesitylene) into the HMF product solution in NMR solvent. The %HMF yield can be calculated by using the integrated values of the aldehyde proton (d = 9.58 ppm) of HMF and three aromatic ring protons of mesitylene (d = 6.79 ppm).
Dear Mustafa,
you can read the article that I attach. In this cas you can have the concentration of furans with two analysis, the first one at 284 nm the second one at 320 nm. There an equation to establish the final concentration. I hope that is helpful for you.
Sincerely,
gaetano
Dear All,
Thanks for all the information. Today I also tried to establish the correlation to determine HMF concentration in isopropanol. The max peak height is not at 284nm but shifted to 306-312 depending on concentrations. Any advice regarding this would be highly appreciated. Another point is that the intensity is too high (over the limit of the device at 3A) at very low concentrations around 0.005M.
Thanks in advance,
Anh
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