Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Where can I find a reliable and updated methodology related with the design of spiral heat exchangers?

I need to design an spiral heat exchanger, buy I don't know what reference or bibliography (preferably a book) should I consult in order to get an updated and reliable methodology related with...

14 February 2021 7,910 3 View

Which are the factors that determine in a tourist the destination for their travel?

I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).

09 February 2021 314 7 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Can anyone recommend me a NMR Handbook?

Hi, I've been searching for a NMR Handbook with 29Si chemical shifts information but I can't find one. Also I've tried to find an online database with that kind of info but I have no lucky. Can...

02 February 2021 307 3 View

Equation to calculate Chlorophylls and carotenoids concentrations from a MeOH:HCl 1% (v/v) plant extract solution?

Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....

25 January 2021 3,686 2 View

Cronbach's alpha or McDonald's omega? Which would be more correct and in what cases?

Cronbach's alpha or McDonald's omega? When it comes to reliability, we find different methods for its estimation. Immediately, the name of Cronbach's Alpha coefficient arises, as it is the most...

21 January 2021 330 4 View

Which aqueous solution should I test to make positive NPs stable to get mayor zeta potential +40 mV?

Hi, I need to find the correct media to make my positive charge NPs stable in an aqueous media. I have tried different solutions at different pH but the zeta potential does not increase more than...

21 January 2021 6,490 6 View

Mysterious line across SDS gel?

Does anyone have the similar issue? A line across the denature SDS gel shows up after staining with Coomassie blue and there are many lines in every lane if you take a closer look. This gel had 15...

07 January 2021 5,610 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How can we estimate sample size for a moderated mediation analysis with more than two mediators?

How can we estimate sample size for a moderated mediation analysis with more than two mediators? I am looking for scripts, macros, syntaxes, that allow us to estimate sample sizes for research...

15 February 2021 6,813 3 View

In Abaqus, is it possible to remove elements which are too complex for meshing from a sequence obtained by getByBoundingBox() ?

Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...

03 February 2021 8,198 1 View

How, if at all, can R scripts/packages be used to perform cluster analyses and motif analyses on digital trace data?

02 February 2021 728 3 View

Unbalanced panel data fixed fir effects and industry-by-year fixed effects. Is plm package suitable? or lme in rstudio?

I am not sure if an unbalanced panel data set doesn't give me good results. Do I have to have all years of my panel data? How do I select the variables? I only select those where I have no missing...

30 January 2021 7,258 6 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View