I injected carrageenan on mice hindpaw to evaluate neuropathic pain and inflammatory response. After RNA extraction (by Trizol), reverse transcription and real time PCR, only the control group (saline injected, without carrageenan) had positive amplification.
Since carrageenan is interfering with my reactions, is there a safe way to get rid of it?
Dear Lucas,
I know my answer comes quite late. I have seen that RNA extraction is performed in Chondrus crispus with RNeasy Plant Mini Kit from QUIAGEN (Kowalczyk N, Rousvoal S, Herve C, Boyen C, Collen J (2014) RT-qPCR Normalization Genes in the Red Alga Chondrus crispus. PLoS ONE 9(2): e86574. doi:10.1371/journal.pone.0086574). As this is the algae from which carrageenan is mainly extracted, it may worth to give it a try. I will like to ask you if you managed to find any solution for cleaning up your RNA from carrageenan as I may be experiencing a similar issue.
Kind regards,
Adrian
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