I am interested in a mouse model of a controlled cortical impact that would mimic sports concussions by creating a mild TBI without any incisions, but I'd like to try and create specific coordinates and target specific brain areas. I figured I could use the eye as a landmark to find Bregma, but I can't seem to find an atlas for the head like you'd find for the brain or skull. Is there a reference for the average mouse skull size with measurements like the mouse brain atlas, or another effective landmark for locating Bregma without creating an incision?
By "closed mouse skull" I assume you mean that the skin on its head is intact. If you cut the skin open is pretty obvious where the bregma is just by looking at the skull. If the skin is intact, the bregma is located between the eye and the base of the ears, with a closer proximity to the eye. There is no exact measurement because the older the mouse is, it moves a little to the posterior side.
Bregma is actually based on skull anatomy: the crossing of the coronal and sagital sutures. Hence, you can find it without opening the skull.
However, if you dont want to make a skin incision. The bregma is located between the posterior part of the zygomatic arch *which you can feel under the skin and muscle.
Thanks for your responses, Louis-Charles Béland and Demos Kynigopoulos. You are both correct in assuming I meant finding Bregma without making an incision. I clarified the wording of my question to reflect that, so hopefully it makes a little more sense. Those are both great suggestions and I'll take those into consideration as potential options.
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