I ran an experiment in order to analyse the growth of a Pseudoalteromonas strain. I made an overnight culture and after 17 hours of incubation I began OD reading every hour, with every OD I also made a serial dilution spot plate (10^1-10^8) with 3 spots (20 microlitres per spot) per dilution and left each plate to incubate overnight. The next morning I checked on my plates and only my first 3 dilutions had significant growth (but was too numerous to count). Any information on this problem would be greatly appreciated.