How to find standard axial compressor blade data files ?

I'm looking for standard axial compressor blade row data files for CFX simulation , so could somebody tell me where can I find them ? Like rotor37 , PBS rotor , and something else about them...

13 February 2021 7,745 3 View

How to do ORR test with pine(Which means RDE or RRDE electrode)?

I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...

14 January 2021 1,869 2 View

Why fluorescence signal drops during thermal shift assay?

I am performing a TSA to investigate binding of a ligand to the protein by performing a serial dilution of ligand against same conc. of protein (~2uM). Interestingly at low ligand conc the...

02 August 2020 3,167 1 View

What kind of mass movement is this?

While I'm working on a project about landslides on the Tibet Plateau, I encountered many large and valley-filling deposits that seem to be some kind of mass movement but cannot find their source...

30 July 2020 6,134 5 View

Can anyone help me about probability sampling?

Now I am doing my DBA research, the whole population are the public university teachers in one of Chinese 's province, there are 22 universities. and I want 400 samplings, the total population is...

23 February 2020 248 3 View

How to generate nucleotide and amino acid sequence in same figure just like cloning journal sequence?

Hi all, I am writing manuscript for cloning research, I want to ask if there's any software that allow us to generate nucleotide and amino acid sequence in one same figure, I have been doing using...

15 January 2020 6,290 3 View

How to separate ester from carboxylic acid by using chromatography?

I want to separate my target compound, 1, 3-phenylene bis[4-{[4-(pent-4-en-1-yloxy)phenyl]diazenyl}benzoate (ester) from the reactant 4-{[4-(pent-4-ene-1-yloxy) phenyl] diazenyl} benzoic acid. I...

05 January 2020 1,836 10 View

Did someone read the papar about the relationship of creative climate and creative self-efficacy?

What do you think of the relationship of creative climate and creative self-efficacy is? Please share some opinions of yours. And if you have any paper to recommend, I'd be very grateful. Thanks...

20 December 2019 1,951 3 View

How to study the influence ot feedback on creative self efficacy?

I want to study the inflence of feedbcak on creative self-efficacy. And this is an activity of students. They will receive feedback after this activity. But the question is the feedback is given...

16 December 2019 7,526 3 View

Does anybody know about how to develope the SUSTAIN model by USEPA?

we are considering to develope the SUSTAIN model adding some new functions. the officia website on USEPA applied the source code of its interface and simulation engine, but it's an extension tool...

05 September 2019 4,312 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View