Absolute Quantification: Serial dilution resulted in same CT values?

Hello, we are currently working on absolute quantification with SYBR Green qPCR. We excised from a plasmid containing the cDNA, a fragment, which will be amplified by the primers we used in our...

10 November 2020 769 4 View

Healthy cell comparison for breast cancer and prostate cancer?

I am trying to find the most acceptable healthy cell line to compare drug cytotoxicity against breast cancer and prostate cancer cell lines in vitro. Any assistance would be appreciated.

18 October 2020 3,730 3 View

How to sterilize 17-beta estradiol?

I am planning on making working solutions of 17-beta estradiol in DMSO. The drug has been exposed outside of the culture hood. My studies will last up to 7 days, so I am wondering if I should...

08 July 2018 7,845 4 View

What is the best way to differentiate between efferent and afferent sympathetic nerve fibres immunohistologically?

Efferent as afferent renal sympathetic nerve fibers have a important relevance in the development of cardiovascular disease. Thyrosine hydroxylase is a marker for sympathetic fibers. Is there a...

04 June 2018 4,657 2 View

Is it important to have specific and detailed goals and objectives for application in the management of fish species?

Most fish management actions rely mostly on current and recent harvest data to set future harvest levels. Should this be improved by including metrics such as desired age/size range, average...

31 December 2017 4,139 0 View

Hi how do you use purified antibody in flow cytometry?

how does it differ from conjugated antibodies? I used PE or FITC conjugated antibodies before but in this time, there is no available conjugated ab for this specific protein I want to measure. I...

10 April 2016 5,041 3 View

How can I best analyze the change of an insect community composition and diversity over time?

The communities abundances were measured under 3 different treatments, over a time period of 55 days.

06 December 2015 8,027 8 View

How do I treat the transition between excitonic absorption and the Urbach tail?

Dear colleagues! I am wondering if there is any method to properly treat the transition from absorption by gap states (Urbach tail) to excitonic or band-to-band. When modelling all contributions...

29 September 2015 2,731 0 View

What causes Vitamin B1 (Thiamine) deficiencies in native predator fish that rely on a large percent of exotic prey fish in their diet?

08 September 2015 6,268 3 View

Has anyone experienced problems with drying THF and diethyl ether using a solvent purification system (MBraun)?

We are experiencing problems drying THF and diethyl ether with an MBraun SPS solvent purification system. The solvents have the expected low water content (10-20 ppp according to...

03 July 2015 8,464 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View