Is there a method to measure the (ATP-dependent) activity of SWI/SNF remodelers in cells/cell lysates (in vitro)?

16 June 2013 1 10K Report

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Check out some of the older work from Peter Becker and Patrick Varga-weisz. I believe the early work on CHRAC used fairly crude nuclear extracts to follow ATP-dependent nucleosome mobilization. There is no way to really look at a particular enzyme without at least an IP.

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