Is there a method to completely remove OCT from frozen tissue?

25 June 2020 1 2K Report

I am trying to remove OCT from frozen tissue for DNA/RNA sequencing. My goal is to remove a portion of tissue from the OCT embedded tissue, without thawing or greatly disturbing the remaining tissue, and then using that portion of tissue for DNA/RNA sequencing.

Any help or suggestions are appreciated!

Thank you!

Ismael Hernández Núñez

If you leave OCT at room temperature, the OCT will unfreeze, however I do not know what will be the state of this DNA/RNA... However... You could try. Good luck!

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