Dear Daniel,

I found a paper about ATP analysis for luminometric assay. They used 0.2 M NaOH/0.5 mM EDTA to extract ATP. I hope this information may be helpful. please find the attachment for the paper.  huiyu

This is another paper about Luminometric assay of ATP

ATP concentration was determined with the Luciferin-Luciferase method as described previously (Ronner et al., 1999); in brief, the assay solution was prepared as follows: 250 mM glycilglycine, 2 mM EGTA, 2 mM MgCl2, 0.4 g/liter BSA fatty acid free, 7.5 mM DTT, 15 μM luciferin, and 10 μg/ml luciferase. Cells were lysed with the ATP lysis buffer (0.2 M NaOH and 0.5 mM EDTA), and an aliquot of the obtained extract was diluted with the ATP dilution buffer (0.1 M NaOH and 0.5 mM EDTA). In the luminometer, 20 μl of this mixture was added to 100 μl of the assay solution, and the ATP content was measured. Data were expressed as nanomoles of ATP per milligram of protein (Bradford, 1976).

please link the website: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2173682/

For determining ATP you don't need to deproteinize the samples. You just need to open the cells and inactivate ATP degrading enzymes. As the light emission varies with the composition of the sample matrix it is in general necessary to calibrate each assay by measuring the light Before and after adding a known amount of ATP standard (cf. attached paper). We have several kits for this type of assay. Send an email with the details of your samples and I will be happy to provide free of charge technical support.

Best regards,

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