The assay from Essen Bioscience is very expensive and I am looking for an alternative. Any help is welcome!
For cytotoxicity you can use SYTOX Green, for viability you can use Calcein AM. I'm not sure how the cost compares.
Does anyone know of an ACTH receptor (MC2R) positive cell line? So far I have not been able to find a readily available cell line expression this receptor and that is responsive to ACTH.
06 July 2016 399 4 View
We have cell lines that were obtained from patient tumors. We would like to validate their origin using a cell line authentication service or similar. Does anyone have experience with this?
09 October 2013 3,888 13 View
Can anyone recommend how/where I can get human primordial/fetal germ cells that I can grow in culture and that can be differentiated into testis cells?
07 August 2012 4,859 2 View
Does anybody have any experience with quantifying nuclear EGFR (or any other nuclear protein) using FACS analysis? The EGR is normally, and far more abundantly located on the plasma membrane. I am...
04 May 2012 4,307 10 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View