The assay from Essen Bioscience is very expensive and I am looking for an alternative. Any help is welcome!
For cytotoxicity you can use SYTOX Green, for viability you can use Calcein AM. I'm not sure how the cost compares.
Does anyone know of an ACTH receptor (MC2R) positive cell line? So far I have not been able to find a readily available cell line expression this receptor and that is responsive to ACTH.
06 July 2016 325 4 View
We have cell lines that were obtained from patient tumors. We would like to validate their origin using a cell line authentication service or similar. Does anyone have experience with this?
09 October 2013 3,794 13 View
Can anyone recommend how/where I can get human primordial/fetal germ cells that I can grow in culture and that can be differentiated into testis cells?
07 August 2012 4,774 2 View
Does anybody have any experience with quantifying nuclear EGFR (or any other nuclear protein) using FACS analysis? The EGR is normally, and far more abundantly located on the plasma membrane. I am...
04 May 2012 4,203 10 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View