Hi,

I am trying to stain different cellular compartments in bone marrow sections from adult mice. When I use direct labelled antibodies, for example CD3-FITC, CD9-PE etc, the staining result is always very bad. I can not see any specific signal. But when I try with primary and secondary antibody, I usually get good result. Curretly there is an experiment that requires to use direct label antibodies. Therefore, I am wondering if there is any good way for me to stain bone marrow sections using direct labelled abtibodies. Thanks.