I need fluorescent markers for the liver endothelial cells. I have used anti-Pecam antibodies and they worked well, but something else that shows endothelial cell function and health would be great.
Hello,
it is very difficult to answer your question, because except microscopic analysis (for fenestration analysis), there is no highly specif marker of this cell type. You could read the article of March et al. Hepatology 2009, it could help You, because as wrote Thomas MOhr, in in vitro study (in 2D) sinusoidal specific phenotype can be lost. This phenotype can be highly different between in vivo and in vitro conditions. For information about markers potentially expressed in these particular conditions read Am Elvevold J Physiol Gastrointest 2008
Hello,
it is very difficult to answer your question, because except microscopic analysis (for fenestration analysis), there is no highly specif marker of this cell type. You could read the article of March et al. Hepatology 2009, it could help You, because as wrote Thomas MOhr, in in vitro study (in 2D) sinusoidal specific phenotype can be lost. This phenotype can be highly different between in vivo and in vitro conditions. For information about markers potentially expressed in these particular conditions read Am Elvevold J Physiol Gastrointest 2008
Thank you all very much for such interesting remarks!
I will read the references you provided thoroughly!
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