MCM2 is a good one. (Ref: DOI: https://doi.org/10.1523/JNEUROSCI.4608-03.2004 )

Thank you very much Emily! I´ll give it a try!

Hi,

I found your question very interesting because you want an antibody that identifies neuronal progenitors, but not glia. Neuronal progenitors are glia. I wonder what you expect this antibody to mark. Can you explain it to me? Just curiosity.

Thank you

Hi Daniel,

As you said, neural progenitors express GFAP and in the adult brain can be found as astrocytes in some areas (SVZ, Hp and Olf). However, a more differentiated state of the neural progenitor can lose this phenotype. An immature neuroblast can express DCX and before Nestin. But, I think, what he is asking for is a marker that could be expressed in a stage more or less like a Nestin-expression one but it is not found in the astrocytes.

Regards,

Jordi.

Jordi Galiano-Landeira

Thanks for your comment. I would be very interested in finding out if that is possible. From the knowledge I have from glia, you can identify immature neurons, because they express glial markers (Gfap, Nestin, Vimentin, DCX .. etc). When they are not, they are not immature anymore. Indeed, the cells you mention in the SVZ, HP and Olf, are not astrocytes, but radial glial and NG2 cells expressing Gfap.

Regards,