What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,005 2 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,226 1 View

Transfection in HFL-1 cells?

I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...

17 November 2019 484 2 View

I have putted the 10fold amount of 2-Mercaptoethanol in my stripping buffer by mistake. Does anyone have experience with such a mistake?

I think my proteins on the PVDF membrane are gone, because there is nothing to detect anymore. But on the other hand the membranes smell after a lot of washing procedures still of 2-ME. Maybe my...

18 July 2018 171 3 View

Gaussian16, dG solvation free energy?

Is there any keyword missing to get an idea of the dG solvation free energy of a molecule? # M062X/6-31G* Opt SCRF=(SMD,1stVac,Solvent=water) From what I understand from the gaussian09 user...

25 March 2018 1,196 3 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

Any advice out there for using CRISPR/dCas9?

I'm working on a project using CRISPR/dead Cas9 for gene expression. I've seen a lot of discussion and recommendations here for using CRISPR in gene editing or knockouts, but not so much regarding...

21 September 2015 9,615 5 View

Can anyone provide advice on how to model age captured in categories within SEM?

10 July 2015 4,972 15 View

How can I regenerate a Pt surface after polymerization?

I have a Pt crystal I would like to reuse. The Pt surface has been electrochemically grafted with a diazonium salt and subsequently polymerized with GMA. I'm thinking it should be possible to...

09 July 2015 5,149 2 View

Is there any recent work on literary language (or the nature thereof) or of "literariness" more generally?

Critics don't seem to be interested anymore in distinguishing between ordinary and literary language, but I'm wondering whether this is a crucial issue we need to return to.

06 October 2014 8,200 5 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View