Hi,

I used Alexa Fluor 350 conjugate of NeutrAvidin biotin-binding protein. I used it for looking at matrix proteins.

Jackson ImmunoResearch  has some of the better simple conjugates and has a variety of fluors depending on your needs, I like them because they are a little more cost effective.   I can't give  any advice on a protocol as concentration/timing varies according to your assay.  I have also used the NeutrAvidin binding protein above, it also works. So you are aware that streptavidin is quite large and may have an affect on the cells ability to endocytose your extracellular proteins?

Thank you everyone. 

I've used Jackson IMmuno's SA-Alexa Fluor 680 to image biotinylated lysate on a LICOR NIR blot scanner. 1:20000 dilution would be a good starting point for loading 30ug of lysate/lane. The biotinylation was done with Thermo's Sulfo-NHS-SS-Biotin.