Looking for a list of anatomical regions that are very likely to be affected by an occlusion of the MCA in mice.
Hi Felix,
I think the answer to your question is more or less trivial: Stritaum. Depending of ischemia time and injury, somatosensory cortex.
I am isolating exosomes from human plasma using the IZON SEC column. I want to detect exsomal markers Flotilin-1, CD9, HSC70 and TSG101 in my samples. I am using SDS -Page and PVDF membrane, all...
31 December 2019 2,782 5 View
I have been trying to get my ELISA running for a while now and at the moment don't know what else to try. I am trying to detect Flotilin-1 in lysates of human monocytes. Primary antibody is...
11 December 2019 1,313 3 View
As the myokardium itself is not or hardly innervated by afferent nerves, I wonder how the pain in a heart attack is generated? Is it mediated by visceral innervation of the coronary vessels or by...
03 April 2014 1,572 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View