Is there a consensus in the stroke research community on which regions are mainly reliant on the MCA in mice?

02 February 2019 1 7K Report

Looking for a list of anatomical regions that are very likely to be affected by an occlusion of the MCA in mice.

Hi Felix,

I think the answer to your question is more or less trivial: Stritaum. Depending of ischemia time and injury, somatosensory cortex.

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Felix Knab's questions See All

How to optimize Western Blot of exosomal markers?

I am isolating exosomes from human plasma using the IZON SEC column. I want to detect exsomal markers Flotilin-1, CD9, HSC70 and TSG101 in my samples. I am using SDS -Page and PVDF membrane, all...

31 December 2019 2,630 5 View

Why is my ELISA not working?

I have been trying to get my ELISA running for a while now and at the moment don't know what else to try. I am trying to detect Flotilin-1 in lysates of human monocytes. Primary antibody is...

11 December 2019 1,192 3 View

Why does a heart attack hurt? How is the pain mediated?

As the myokardium itself is not or hardly innervated by afferent nerves, I wonder how the pain in a heart attack is generated? Is it mediated by visceral innervation of the coronary vessels or by...

03 April 2014 1,475 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View