Felix Knab

4 Questions 3 Answers 0 Followers

Questions related from Felix Knab

How to optimize Western Blot of exosomal markers?

I am isolating exosomes from human plasma using the IZON SEC column. I want to detect exsomal markers Flotilin-1, CD9, HSC70 and TSG101 in my samples. I am using SDS -Page and PVDF membrane, all...

01 January 2020 2,726 5 View

Why is my ELISA not working?

I have been trying to get my ELISA running for a while now and at the moment don't know what else to try. I am trying to detect Flotilin-1 in lysates of human monocytes. Primary antibody is...

12 December 2019 1,269 3 View

Is there a consensus in the stroke research community on which regions are mainly reliant on the MCA in mice?

Looking for a list of anatomical regions that are very likely to be affected by an occlusion of the MCA in mice.

02 February 2019 7,022 1 View

Why does a heart attack hurt? How is the pain mediated?

As the myokardium itself is not or hardly innervated by afferent nerves, I wonder how the pain in a heart attack is generated? Is it mediated by visceral innervation of the coronary vessels or by...

04 April 2014 1,539 4 View