I need to find some compound that stains the Golgi apparatus and that can be used in live cell microscopy. I cannot transfect a vector because I already have to use another one to stain other organelles. Do you have any ideas?
It should be possible to do a co-transfection of different vectors mixed together to stain each organelle. Depending on what channel you are looking for, NBD-ceramide might be useful, which can be visualized relatively well using a GFP filter set. http://www.lifetechnologies.com/order/catalog/product/N1154
If you are looking for another channel, I am sure there are other dyes conjugated to ceramide that can label the Golgi for you.