I am looking for nuclease deficient Cas9(dCas9) and the CRISPR RNA on it for my academic studies.
Hi there,
Addgene sell this plasmid: https://www.addgene.org/46569/
You can clone in your own crRNA that you desire. I hope that helps you!
I haven't seen any commercially available purified dCas9 out there. I think your best bet is to either use a plasmid, as Simon suggested, or clone the gene under a T7 and make mRNA of dCas9.
If you're looking for recombinant protein - a number of companies seem to sell it (see below for examples). A simple Google search found these in less than 30 seconds...
If you are looking for plasmid expressing dCas9 then follow Simon's link - Addgene is the goto place for all sorts of plasmids nowadays and really can't beat the price!
http://pnabio.com/order.htm
http://www.novateinbio.com/en/recombinant-proteins/1156796-dcas9-d10a-h840a.html?gclid=CK2Tq9CF59ACFe-97QodvPoBsA
http://www.excellgen.com/cas9-d10a-h840a-dead-recombinant-protein-nls-dcas9-19633.html
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