Apart from GC-MS analysis which method would be best to quantify terpenoids from plant extract?
Simple calorimetric method
Good luck to you
Thank you sir that was very helpful. I just had one doubt, would this method work for monoterpenoids as well?
Dear Alagappan
Check this publication.
http://www.nature.com/protocolexchange/protocols/2529#/procedure
Check the following threads for other methods
https://www.researchgate.net/post/what_is_the_best_solvent_system_for_isolation_of_terpenoids_and_flavonoids
https://www.researchgate.net/post/Do_anyone_has_protocol_of_simple_method_for_spectrophotometric_estimation_of_phytochemical_component?_tpcectx=profile_answers
@Dr Murugesan Muthu , Sir may I know what is the principle behind the colorimetric assay that you suggested?
I think colorimetric analysis is not possible because they don't absorbe in UV at least they have many conjugate double bond. The others way is to derivative to an ester compound or use HPLC with refraction index as detector.
Check this interesting link on phytochemistry:
http://www.slideshare.net/rahulbs89/extraction-of-plant-contituents
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