Hi Henning

have you considered sucrose for clathrin dependent endocytosis? or the drug dynasore -dynamin inhibitor...

Cheers

Jurjen

Thanks dude!!! Googling for your suggestion dynasore, i found this publication:

doi: 10.1242/jcs.038729.

"A number of compounds have been shown to inhibit clathrin-dependent endocytosis. These include methyl-β-cyclodextrin (β-CD) (Rodal et al., 1999), phenothiazines (Horwitz et al., 1981; Kuratomi et al., 1986; Salisbury et al., 1980), monodansylcadaverine (MDC) (Schlegel et al., 1982), chloroquine (Wang et al., 1993), monensin (Dickson et al., 1982), hyperosmotic sucrose (Hansen et al., 1993) and dynasore (Macia et al., 2006; Nankoe and Sever, 2006)."

I ordered a couple of them. We will compare what works best. I will post here the outcome...

Cheers

Henning

Sure thing!!

Good luck!

Jurjen

It depends on the specific receptor protein and the endocytic mechanism operating on the given receptor. Reading the result of your Google search, I notice for instance that ...methyl-β-cyclodextrin (β-CD) (Rodal et al., 1999) is listed as an inhibitor of clathrin-dependent endocytosis. Indeed, but it works in the opposite direction for other receptors... (see e.g. Kumari, S., Borroni, V., Chaudhry, A., Chanda, B., Massol, R., Mayor, S. y Barrantes, F.J. (2008). Nicotinic acetylcholine receptor is internalized via a Rac-dependent dynamin-independent endocytic pathway. J. Cell Biol. 181, 1179-1193). 

Best wishes

Francisco

Thanks Francisco. If it does not work with one of the earlier mentioned agents, we might have a clue why... Like I said. I will check and post here the outcome....

Overexpressing Dynamin I-K44A mutant (defective in GTP binding) could also be an alternative solution in your case to inhibit CCV-mediated endocytosis.

http://www.ncbi.nlm.nih.gov/pubmed/12011079

Thanks for your answers. Unfortunately, neither cooling down nor overexpression of dynamin I mutant is an option...