How can one quantify & document indigenous mycorrhizal fungal spores in soil in an area?

In most part of Nigeria little or no research has been carried out on documenting the indigenous mycorrhizal fungi present in soils around the country. If one is to carry out this researches, what...

07 August 2019 6,309 4 View

Can salinity affect the proximate composition of plants?

In my analysis of some vegetables grown under salt stress, i observed irregular patterns in the concentrations of proximate compositions of these vegetables. what role does AMF fungi play in such...

09 October 2018 3,136 0 View

What is the best method for carrying out a survey of indigenous mycorrhizal species in soils of a particular area?

answers should include appropriate sampling methods and sample size, experimental design, proper documentation methods etc. additional and necessary information and pdfs will be appreciated. thank...

06 July 2018 8,464 2 View

Is there a comprehensive guide/manual for mycorrhizal species spore identification?

I need a comprehensive guide/manual with pictures and illustrations which i can use to identify the spores of the different species of mycorrhizas from soil samples. isolation methods will also be...

06 July 2018 9,825 3 View

What is the reason for reduction in organic carbon, total nitrogen and phosphorus in saline soil?

I'll really need detailed effect of salinity on soil physico-chemical properties. attached materials will be appreciated.

04 May 2018 4,258 10 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View