Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

Genus name priority from the same publication (Zoology)?

I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...

19 January 2021 1,920 4 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

Trizol samples wont freeze. Can it cause problems?

Hi, everyone. I recently lysated cell culture with Trizol reagent, and I put my samples in -20C. But, some of my samples wont freeze, and I can see two separete phase appearing in them. I'm awaer,...

06 November 2020 10,002 1 View

How to amplify lignin degrading gene?

Hello colleagues, I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are...

20 October 2020 9,654 3 View

How to remove abnormal behavior at support of FE model of RCC Beam?

Hi, I am modelling a half model of RCC beam in ANSYS Mechanical APDL. The model I have created is exhibiting abnormal behavior at the support with abnormal cracks. I would be grateful if you...

12 October 2020 8,596 3 View

How to calibrate the parameters of coupled damage-plasticity microplane model of concrete for FE modelling of RCC beam in ANSYS Mechanical APDL?

Hi, I am trying to model an RCC beam in ANSYS Mechanical APDL. I have the value of Compressive strength, and modulus of elasticity of the concrete of the experiment I am trying to validate ? Can...

28 August 2020 4,623 2 View

Culturing CHO-freestyle cells without shaking?

Hi everyone! Recently I started working with CHO-Freestyle suspension cells, but our laboratory does not have an orbital shaker. I tought it would be okay without it, so I just used a normal T25...

20 July 2020 4,597 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

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I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View