Is the PCA tool in ArcGIS spatial analyst a Geographically weighted PCA?

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How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

I see GFP signal on cells even though there is no GFP in them. Can anyone tell me what could be the reason?

These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...

06 June 2024 2,289 3 View

Can anyone help me with GCF (Gingival Crevicular Fluid) elution from PerioPaper?

I am using PerioPpaer from Oraflow, to collect GCF, 8-12 PerioPapers from each subject and a pooled sample in PBS elution buffer. For the elution, I am vortexing for 1 min, then slow...

03 June 2024 9,767 0 View

How to cal culate the direction of dipolemonet for a molecule energy optimized using Gaussian package?

I performed single-point energy optimized using Gaussian software, followed by mapping the electrostatic potential surface around that. There is a clear dipole for that molecule but I want to...

24 May 2024 8,549 1 View

Could anyone kindly provide me the data regarding the variation of water flow rate for 24 hour horizon for small hydro power used in multi period OPF?

Optimal power flow, small hydro

20 May 2024 7,590 0 View

How to calculate the angular velocity of a rotating cell from a microbe video?

I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...

18 May 2024 4,289 3 View

I have docked 17 antiviral drugs using UCSF Chimera AutoDock Vina. I need to select the best 3. How do I do that?

I need to select 3 best results from the list of the 17 antiviral drugs. How do I select that?

25 April 2024 5,386 1 View

What type of seagrasses and saltmarsh grasses are found in coastal odisha?

explain its morphology, taxonomy and habitat distributiion

03 March 2024 1,293 1 View

Can we extract and use only the sequences from the aligned regions for constructing phylogenetic tree and delete the unaligned sequences?

Suppose we are performing a phylogenetic analysis among different fungal species and we wanted to see how closely our species of interest is related with the other species across different...

28 January 2024 9,895 4 View

How to increase bootstrapping values of a phylogenetic tree?

I want to establish a phylogenetic relationship of our fungal species of interest along with the other fungal species belonging to the same order (Hypocreales). In order to perform this, we have...

22 January 2024 9,174 5 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View