Is the Ni, Cd, Pb and Hg concentration in packed blood cells higher than in serum, or viceversa?

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Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

No title

Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...

06 December 2020 3,504 4 View

Problems with fluorescent bioconjugation of short peptide?

I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...

02 August 2020 2,775 3 View

What is the experimental set up/or protocol to measure distribution of the light emitted?

Hello, Could anyone here please tell me the experimental set up, or the protocol to be able to measure the angular distribution of the light emitted (fD) by some OLED device? I have seen that...

10 February 2020 7,932 2 View

How to measure HRV-PRV agreement using Bland-Altman plots from Excel (step-by-step guide)?

Hi! I recently installed the Real Statistics complement for building Bland-Altman plots in Excel. However, I don't know how to use it in evaluating the agreement between HRV and PRV series. Could...

25 November 2019 3,736 3 View

What is the minimum number of PP intervals required for accurate and reliable extraction of Pulse Rate Variability (PRV) from PPG signals?

The minimum number of RR intervals required for reproduce several metrics derived from HRV (e.g., HF power) can be found in the literature (Richards et al., 2010). However, what about PPG signals...

02 June 2019 353 2 View

Any fast publishing Scopus indexed journal ?

Who can help with a link to the a fast publishing scopus indexes journal. I am looking at a journal that will respond in like 3-5 weeks

19 August 2018 1,327 18 View

Metagenomics databases. Which one choose?

In bioinformatics databases play an important role in the results, whether chimera detection or OTUs assignment (16S metagenomics). In your experience, which databases have a better relation...

11 January 2018 858 3 View

Meshing Advise for 2 phase flow ANSYS FLUENT simulation?

I am having difficulty meshing the attached geometry for a two phase mixing type application where I care about the volume fraction at various points along the tubes. I have defined 3 bodies Body...

05 December 2017 1,306 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View