Hello,
I am wondering if the molar extinction co-efficient (molar absorptibility) of a compound is same in UV as well as in fluorescence light. If there is any differences then what the extent of it and what could be the possible reasons? Your idea and suggestions are always welcome.
Thanks in advance.
What do you mean by fluorescence light?
I guess you mean visible light.
If the chromophores you are looking at are different then the absorbance of the sample will change because the extinction coefficients will be different.
The change in absorbance will be related to the ratio of the extinction coefficients since the concentration, path length and all other factors being the same.
I'm going to interpret your question as asking whether the molar extinction coefficient for absorbance, as used in UV-VIS absorbance spectroscopy to measure the concentration of a chromophore, has the same value for the fluorescence excitation spectrum. The answer is yes, as long as the two measurements are made in the same solvent and at the same temperature. The molar extinction coefficient is a property of the chromophore and its interaction with the solvent.
Hello Dr. Ravi and Dr. Adam,
Thank you so much for your information.
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