Is the methylation process of phospholipids necessary for RP-HPLC purification?

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Jasim Al-Saadi

You Do not need Methylation for phospholipids determination .Reversed-phase HPLC offers a method for separating the phospholipids of each class (PC, PE, etc.) into its individual molecular species, often with baseline resolution, short columns, and isocratic solvents. In general, the retention time increases with increasing chain length and decreases with increasing unsaturation regardless of phospholipid class

Andrei Blasko

No, use RPLC with Corona Charged Aerosol detector (see some of my publications on CAD) and use the newest preparative column technology on the market.

Karen A. Darbinyan

No need methylation for RP-HPLC of phospholipids.