I want to produce a Bacteria that belongs to Bacillus species at large scale so can i use LB medium or is there any other suitable medium that can be used with ease. I need your valuable suggestions. Thanks
It depends upon for what purpose you are going to multiply the bacteria. If you are interested in only multiplying the bacteria then you can use LB media or any suitable growth media. But if your purpose is to obtain the products like extracellular enzymes ( you have mentioned fermentation so0 then you have to use specific media.
I hope this will help
It is widely used medium for mass production of Bacteria, however, it is also related to the purpose for which the multiplication is required.
You should also add Glucose, and 10mM of Arginine to the medium to avoid sporulation.
LB medium is a general medium. You need to know the nutritional status of your organism and try different media, growth conditions and use the one in which you get more growth if you grow for its biomass.
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