In a company protocol it was written that sample needed to be centrifuged at 14000 g for 10min. We used 14000 rpm for 10 min. I am confused that is g can be equal to rpm?
Probably it is not like that. Since the sample is centrifuged, it undergoes a circular path and faces a centrifugal acceleration equal to (angular velocity)^2 * radius of circular path. This is then normalized with respect to g (acceleration due to gravity) because the orientation of the sample changes during the test.
So if it is 14000g, then the centrifugal acceleration is 14000 times g. So, if it is 14000 rpm, then it could have a radius of circular motion of nearly 0.0639m....may be
g depends of the radius and RPM. You can use the site below for transformation.
http://insilico.ehu.es/mini_tools/rcf_rpm.php
Yes.
In case of precise centrifugation conditions, which must be specified in terms of relative centrifugal force (RCF) expressed in units of gravity (times gravity or × g).
Geo Bhai
No g is not equal to rpm but rpm can be converted to g if radius of centrifuge is known to you. rpm tells you just about speed but g the actual gravitational force, its depends on speed and radius of the machine
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