Why does my MTT Assay remain in purple color and does not change to yellow?

19 January 2021 5,098 4 View

Why is there cell debris are coming after 2nd or 3rd passage of the cells???

Our lab is facing a difficult problem for almost a year. After thawing the cells are okay, but after 2nd or 3rd passage there are debris present between the cells. We use mycoplasma removed cells...

17 January 2021 5,629 6 View

Is there a way to improve the stability of the calcium alginate beads in alkaline pH?

I am working on heavy metal bioremediation using bacterial isolates. I have immobilized bacteria in 2% sodium alginate then cross-linked in 0.2M calcium chloride for 30 min. I have successfully...

04 January 2021 9,094 4 View

Can you help me for generating chicken swarm optimization matlab code?

Multi-objective optimization through chicken swarm optimization technique. Both maximization and minimization problem.

31 December 2020 2,299 4 View

Any good methods for double-stained markers quantifications from tissue sample using computer programs?

How can we best quantify double-stained markers when comparing diseased and control? For example, if we want to stain one marker of smooth muscle cells and compare the expression in diseased and...

21 December 2020 7,389 1 View

Membrane protein Expression Purification: Broad Asymmetric peak on size exclusion ?

Hello, I am expressing an integral membrane protein (MW 51kDa) in Hi5 insect cells. I initially purified the protein by Nickel-His, concentrated it and subjected it to Size Exclusion...

10 December 2020 2,781 5 View

How to Strip off HSQ( Fox 16) after etching?

We are finding a hard time to strip of residual HSQ ( Fox 16) after using it as a mask in etching? We are coating 600 nm thick HSQ and writing with 3000 microC/cm^2 dose at 1 nA current. We are...

08 December 2020 5,452 2 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Can U suggest standard protocols of quantifying total phenolics & total flavonoids using gallic or tannic acid for the former and quercetin for later?

The problem is that everytime getting the flavonoids content much higher than phenolics which may be, but in rare occasion. In phenolics, I am adding100 ul sample with 900 ul dH2O with...

29 September 2020 4,993 6 View

What is the ideal Elution buffer composition for a His tag protein purification to prevent protein aggregation after freeze thaw ?

Hi All, Recently, I purified 8 proteins and stored at -20 with the elution buffer containing 20 mM Tris, 250 mM NaCl and 200 mM Imidazole. I autoclaved the buffer, so I do not know whether the...

25 September 2020 9,814 6 View

What's this isomorphism at play in quantum optics?

I have looked at the time development of the creation and annihilation operators for a mode of the quantized free electromagnetic field. It was assumed that the equations of motion for these...

29 September 2020 8,955 5 View

Do the deterministic polynomiality and non-polynomiality characteristics of this method prove efficiency?

Dear Researchers, I proposed a new method, called Optinalysis, for symmetry detection, similarity and identity measure between two automorphic or isomorphic graphs. I tested the method using the...

10 August 2020 5,020 1 View

How to locate the isomorphous MgZn2 (n, n') phase existing in AA7XXX-T6 using OM/SEM/FESEM imaging ?

How to locate the isomorphous MgZn2 phase existing in AA7XXX-T6 using OM/SEM/FESEM imaging ? The context of the question is - the aluminium alloy has been applied with super plastic deformation ,...

20 February 2020 8,545 2 View

Where can I publish my research paper in Journals free of cost related topic corporate social responsibility reporting isomorphism?

related to Dimaggio and Powell (1983) and Shabana et al. (2016)

31 December 2018 1,921 0 View

How to prove this isomorphism of the 1st Homology Group?

The problem is: Let Z denote the set of all integers. Consider Z/nZ = Zn as trivial G-module. Show that there is a isomorphism between the First Homology Group H1(G, Zn) and the factor...

11 December 2018 1,081 3 View

How to prove a isomorphism between group homology and group cohomology of finite group?

Let Z denote the set of all integers, and let G be a finite cyclic Group. For every ZG-module A, and n=1,2,3... Show that: Hn(G,A) is isomorphic to Hn+1(G,A).

11 December 2018 5,134 3 View

Hello. Are there any references for subgraph isomorphism problem for dynamic query and data graphs?

I found two references: Shearer et al (2001) and Nguyen et al (2010) which propose algorithms for detecting a sequence of graphs in a dynamic data graph. But are there other references for this...

10 November 2018 5,786 5 View

Invariants under *-isomorphisms of von Neumann algebras?

06 July 2018 9,104 0 View

I am looking for someone to work with who is familiar with Similitude, the Chinese Remainder Theorem, and Buckingham Pi Theorem. Suggestions?

I am trying to work on the following problem and I am willing to transfer universities to find a good advisor: We do not need to take the whole of the real numbers to do engineering. Since...

01 February 2018 2,464 4 View

Do strong qualitative probabilistic representations over finite sets constitute order isomorphisms when not unique or an order emebdding without =?

When a complete comparative qualitative probability order defined over a finite but complete power algebras that is complete in the order. \forall events in P(\omega): A

09 October 2017 1,949 0 View