Is the data obtained from simultaneous gas exchange & chlorophyll fluorescence measurement using LI-COR reliable to study photosynthesis & chlorophyll fluorescence in plants? The photosynthesis, stomatal conductance and Ci values I obtained from simultaneous measurement are noisy and as well the values are quite different from the independent measurement.
Yes, obtained data are reliable. However, you have to make sure that your instruments are properly calibrated, read manuals for detail information. I think you are using two different instruments, namely a portable Leaf Gas Exchange System (for measurement of photosynthesis, stomatal conductance etc.); and Chlorophyll Fluorimeter (for measurement of Fv/Fm values). Please read this attached research paper (related with Ethiopian crops) for detail information.
Article Plant growth and foliar characteristics of faba bean (Vicia ...
Article Differential Sensitivity of Pisum sativum L. Cultivars to Wa...
Article IAA-induced alteration in growth and photosynthesis of pea (...
Article Salinity-induced modulation of plant growth and photosynthet...
Article Growth, Water Status, Physiological, Biochemical and Yield R...
Thanks a lot Azamal. For your information, I used Li-cor 6400XT Leaf Chamber Fluorometer for both measurements and the crop I'm studying is Avocado in Israel not in crop in Ethiopia.
You may try to use a check with fresh Vicia faba wich can be raised in few days in a pot. If noise persist you should revise the leaf chamber thightness, by braeathing onthe chamber when closed. Any change in the signal would indicate a leakage in washers.
Yes, i have used in my experiments.... The data is more than 90% reliable
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