Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using Chromotek GFP-Trap Magnetic Agarose beads for Nuclear Protein Purification?

Hi All, I was wondering if anyone had used the chormotek gfp trap magenetic agarose bead kit for purification of GFP tagged nuclear proteins. I am attempting to purify a gfp-tagged protein...

14 January 2020 4,572 2 View

Solutions to selectively break down proteins and leaving polymer chains intact?

In a project, I am required to degrade proteins to a size of 10Kd. This would prevent protein from passing through ultrafiltration and being absent in the resultant. I suggested using SDS,...

27 January 2019 3,300 3 View

Western Blot Signal Looks Like Backround Color?

I am designing an antibody to detect the protein LynA in western blot. Right now I have 8 potential antibodies (SCFVs) that may or may not bind by western blot. I have an apparatus that lets me...

28 November 2018 5,889 1 View

Taqman microRNA Assay Primer dimerisation at exceptionally high levels?

I have been using taqman microRNA assays for 3 years and have recently come across a very serious problem with one particular microRNA. RT-qPCR analysis shows the miRNA to be expressed at higher...

22 November 2018 8,147 3 View

Can I stain intestines with cresyl violet?

Hi everyone, I apologize if this has been asked before. I am running a study with rats and want to collect intestinal samples to look at histologically. Firstly, do the intestines need to be...

18 October 2018 6,288 2 View

Does the populations of argonaute-2 microRNAs and RISC loaded miRNA correlate with total miRNA within cells/tissue?

We've performed Profiling of Ago-2 RIP miRNA in tissue and also followed it with individual RT-qPCRs and showed that the miRNA levels between the 2 were correlated but this is with a handul of...

02 February 2018 1,841 2 View

Can mice reproduce after in utero injections?

I am thinking of doing in utero injections of mice to study the effects of a drug on the embryos. However, we hope to use the female mice again after it gives birth to the injected pups. Is it...

02 January 2017 882 4 View

Why do prices of consumables from the same vendor vary so much between institutions?

Hi, The lab I have been working in recently move from one institution to the other (moved states too!). I noticed that the pricing of consumables varies a whole lot. For instance, in one...

31 December 2015 9,316 0 View

How can I calculate the Performance Coefficient (Cp) from Ansys Fluent?

29 July 2015 1,355 7 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View