In SDS-PAGE, if 2 entire lanes have more intense bands than the rest does this show increased protein expression?

I ran an SDS-PAGE gel of my total cell samples, each expressed in increasing concentrations of a co-factor to see whether this improves expression of the target protein. I coomassie stained the...

28 January 2020 1,772 3 View

Which cognitive biases are most useful for "justifying" teaching research methodology (social sciences)?

I am teaching an undergraduate course in research methodoloy (social sciences) for aspiring teachers and I usually start with explaining, why scientific methods are important to deal with...

24 May 2018 773 4 View

How to calculate standard errors for the model with measurement error in the explanatory variable?

17 January 2018 8,933 6 View

Can you recommend a good R package for VECM and cointegration?

Hi, can anyone recommend R package for cointegration and vector error correction model?. Ideally the package should be able to deal with both time series and panel data? Best regards KB

27 December 2017 5,264 13 View

What is the best way for DNA isolation from older basidiomata (more than 6 months, dry herbarium items) of wood-decaying fungi? A reliable kit?

Work with Ganoderma sp. and Fomes fomentarius.

19 February 2017 5,032 5 View

Model for depression induction in rats?

25 August 2016 3,701 3 View

Does anyone have experience staining unstained molecular weight ladder prior to running gels?

I am currently running native gels. I need a molecular weight ladder between 1200 and 400 kDa. I only found one commercially but it is unstained. How can I stain it prior to running my gel?

13 August 2014 9,362 2 View

Does anybody work with breast cancer cell lines derived from rats?

Should it be the same principle as when you work with 4T1 cells and Balb/c mice?

30 January 2014 594 3 View

How does using literature to teach junior high reading instruction impact learning?

I am seeking articles that support and advocate for the use of the many types of literature available to learners today, with a focus on teaching junior high students. Thanks.

01 January 1970 4,112 1 View

Is there a single, reputable source listing the languages spoken w/in a country and the percentages of people residing in that country who speak them?

01 January 1970 2,328 6 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

What can cause laser beam polarisation fluctuations?

Dear colleagues, We have observed large fluctuations in laser power in our setup and traced them down to fluctuations in laser beam polarisation. Attached is a schematic of the relevant part of...

14 January 2021 7,505 4 View

Data misrepresentation and Ignorance of socio-economic factors?

Do you think the majority of developing, underdeveloped, and even the developed countries lack in capturing the finer elements of behavioral data generated at various interactive and reactive...

13 January 2021 3,241 2 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View