First of all you have to collect experimental information concerning the constant of thermal destruction named k[=] min-1 as a function of exposure time. Then a ln k vs T-1 graph produces a nice straight line with negative sign. One of the classic microorganism used for these purposes is Bacillus sthearothermophillus (of course all of the energy requirements are specific for each microorganism) and here you have a good calculation example: