Greetings! I'm working on two strains (PAO1 and PAK) of the same bacteria, Pseudomonas aeruginosa. I have obtained the growth curves of the two strains separately to have the OD vs CFU/ml curves of their exponential (log) phase. I obtained R- squared values of 0.99, so I think I have had enough accuracy and precision. Surprisingly they differ too much in their growth kinetics. The count of the strain PAO1 is 12.5 * 10^8 in OD=1 and the other strains is 3.6*10^8. In other words, PAO1 is approximately fourfold higher in quantity in comparison with the other strain PAK. Is that logical?!! I supposed they must be very close to each other since they have close size!
Yes, it is normal. I do not know about the particular strains you are using, but usually different strains grow at different rates.
Hope it helps!
David
It is very frequent, it depends of the presence or not of a genetic package of an external DNA incorporated in their genome.
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