Is SYBR safe DNA gel stain also used for staining ssDNA, or is it only used to stain sdDNA?

More Gladys Thalia Cortés's questions See All

Does anyone have plasmidic cDNA (XM_00135201) codifying for Plasmodium falciparum 3D7 heat shock protein (Hsp70) homologue (PFI0875w)?

I need as a control the cDNA 4.1) codifying for Hsp70-2 protein (or BiP): PFI0875w , giI124506906 ( XP_001352050.1). All my life I had been working with this parasite and would appreciate very...

03 April 2015 9,949 0 View

Do any collaborators have experience with helper phages?

I have been working with screening libraries of cDNA prepared in Lambda ZAP-II phages of Plasmodium falciparum. After screening we found 5 positive clones, reactive with monoclonal antibodies. We...

04 May 2014 7,539 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Kimberly Susan Hall Popular answer

I'm guessing you found your answer long ago but just in case: it depends on the type. SYBR Green 1 is for dsDNA and SYBR Green 2 is for ssDNA. That said, SYBR green 1 CAN be used for ssDNA but preferentially binds to dsDNA. More information at the links below.

http://www.lifetechnologies.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe/sybr-safe-faq.html

http://en.wikipedia.org/wiki/SYBR_Green_I

Kimberly Susan Hall

Masood Sepehrimanesh

Dear Gladys

Totally I can say that SYBR preferentially binds to dsDNA, but will stain ssDNA with lower performance. SYBR can also stain RNA with a lower performance than DNA.

Cheers