IHC perfused brain tissue disintegrating following TBS wash on Super Frost Slide?

Hi all, I am using IHC to look at various neuronal markers in zebra finch brain tissue. Finch brains are perfused with PBS and then 4% PFA and placed in a sucrose solution overnight. I then...

01 January 2020 8,400 3 View

Anyone could explain if lentivirus is pathogen?

Because lentiviral vectors are derived from the HIV-1 pathogen, biosafety issues are an important concern. I was asked to indicate whether if lentivirus is animal or human pathogen when I try to...

10 July 2019 2,950 3 View

How to rear aquatic Ceratopogonidae larvae?

11 April 2017 184 13 View

What is the suitable culture condition for culturing cryopreserved PBMCs for 2-3days to obtain maximal viability rate using flow cytometry?

I have been trying to culture cryopreserved PBMCs, but PBMCs with just media alone (after 48 hours) were giving me very low viability rate when acquired on a flow cytometry. The viability rate...

10 May 2015 6,353 18 View

Does anyone have experience with making lentivirus with "Lentivirus Precipitation Solution" from ALSTEM?

We are going to concentrate lentivirus by using this kit, however, I could not find any published reference about it.

18 January 2015 6,150 3 View

Which pipeline or approaches to predict sRNA's in bacteria?

I'm trying to predict some sRNA's (small rna's) which are non-coding rna's in bacteria. I'm following an approach that i have seen in a paper, that uses MAUVE and RNAz to predict them. But the...

07 April 2014 5,769 2 View

Why can't a statistical test of significance (inferential analysis) be applied to a population?

Or can it be?

23 March 2014 120 13 View

Are the quotients of 1/0 and 0/0 the same?

Their quotients in the calculator are both "error". Math books show they are both infinity.

22 March 2014 5,978 67 View

What sampling designs and formulae are commonly discussed in research/ statistics books?

Are these applied by students in their research works?

07 February 2014 6,677 2 View

Is there any study available containing the results of the validity test of its data-collection instrument?

Or is there any study that measures the validity of a questionnaire?

09 January 2014 4,871 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View