Is serum inactivation always recommended for in vitro culture of primary murine B cells?

Abhirami Kannan Iyer @Abhirami_Kannan_Iyer

08 August 2017 1 5K Report

For studying primary B cell proliferation and activation, will it be better to use heat inactivated serum (FBS)?

Of course. Many antigenic peptides including enzymes, complemets, and other aggregated form of complexes that are labile to heat will be precipitated and avoided by spinning down before you add serum to medium. Recently, presence of exosomes (micorvesicular structures released from cells or tissues into the body fluids (serum, urine and saliva etc) further necessitates inactivation.

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