This process is to remove pollen contents to remove all genetic, lipid and protein materials from plant pollen particles to get empty capsules. Has anybody done the reaction with phosphoric acid at high temperature?
Common laboratory borosilicate glassware ̶ often either called 'borosilicate 3.3' or Pyrex® glass ─ is expected to be seriously corroded by concentrated (conc.) aqueous solutions of phosphoric acid above ~120 °C. Use of conc. H3PO4 (> 30 wt%) from ~40 ºC to ~120 ºC is also not recommended, in general, but can be found acceptable after carefully consideration of the particular experimental circumstances involved by experienced users. Below ~40 ºC, corrosion is expected to be small, but prolonged contact of the glassware with conc. H3PO4 should be also avoided, particularly when volumetric glassware is used or when contamination of the contained liquid with ions contributed by glass corrosion must be avoided to any appreciable extent. Besides these generic guidelines, it would be also advisable to refer to the technical literature that may be found issued by the glassware supplier.
Safe, but never Heard of such harsh conditions for so fine an objective
I agree with Teodoro. It would be better to use PTFE; various kinds of bombs exist for acid digestions.
Perhaps it would be worth looking up cold plasma methods which have been used, for example, for isolating the skeletons of butterfly wings; these contain silica, however.
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