I am planning a project to isolate RNA from differing neuronal populations using LCM. Due to logistics and availability of when I can use the machine, it may prove significantly more viable if I am able to cryosection all my samples (around 60 blocks) first and store the slides @-80C for a few months before proceeding to LCM.
In anyone's experience does this have a major impact on quality of RNA extracted from the tissue? Literature on the topic I've found has been conflicting, with some people proceeding straight to LCM post-cryostat and others saying you can leave slides @-80C until needed.
Obviously I will be doing everything under RNase free conditions, taking careful care of contamination and storing cut slides with desicant to remove potential condensation contamination.
Any thoughts or guidance is hugely appreciated.
More info: Blocks are -80C snap frozen pre-frontal cortex human tissue.
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