Is protein quantification necessary for On-cell western?

02 November 2021 1 7K Report

I am woking with type II diabetes and doing assay on L6 skeletal muscle cell line to understand the glucose uptake by using on-cell western method. But I am confused Do i need to protein quantification for On-cell western?

Bilal Ahmad Mir

As per the protocol, protein estimation is needed. I don't run western blots but run a coomassie protein assay to determine protein levels of the samples against standards. In our lab, we have never used western quantification as it is not needed.

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