Hello everyone. I want to perform TPC assay for phenolic content estimation of my honey samples.
However, the only FC reagent available in my lab is from Sigma Aldrich that got 'suitable for determination of total protein content by lowry method' on the bottle label.
Is it still suitable to be used for my TPC assay or should I purchase another one?
Thanks a lot for your advice!
I believe that they are the same. If you go to the web site (merk a different product number) you will find "suitable for proteins determination".
Yes it is the same but different reaction conditions. And this laso clearly illustrates one of the problems of the TPC method which is its lack of specificity.
Folin reagent ( FC) is famous use in TPC determination content. In every standard methods
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