Is microsatellite study is useful for phylogenetic study of cyanobacteria?

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How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Why Spine gourd fruit become red while it is tender ?

Why Spine gourd fruit become red while it is tender ? No attack of insect pest is recorded.

11 June 2024 8,771 0 View

Wheather HYVs are alowed in Natural farming ?

One of the major objectives of Natural Farming is self-reliant farmer and promotion of traditional varieties so that the farmers can use their own seeds. But it is observed that most of the...

11 June 2024 5,696 0 View

HADDOCK score and binding affinity, any relation?

I am working on Protein-DNA/RNA docking and using HADDOCK 2.4 server, but I couldn't understand the relation between HADDOCK score and binding enthalpy (if there is any). I want to find the...

09 June 2024 3,939 4 View

How to estimate uncertainties in values estimated for river morphometric analysis and channel changes using satellite data?

I have estimated various river morphometric parameters using satellite data and DEM for a river basin. Changes in stream parameters such as sinuosity and channel shifts over a period of time was...

08 May 2024 653 0 View

How to perform NRT calcualtion in gaussian?

#rhf/3-21g pop=nboread RHF/3-21G for formamide (H2NCHO) 0 1 H -1.908544 0.420906 0.000111 H -1.188060 -1.161135 0.000063 N -1.084526 ...

05 May 2024 4,500 2 View

Using KMLYP and BHHLYP?

Hi, I am new to gaussian and was wondering how to use KMLYP and BHHLYP in the gaussian input file

28 April 2024 6,606 1 View

How to prepare alcoholic beverage samples for FTIR analysis?

I want to analyze alcoholic beverages using FTIR for SO2 content and other metabolites. Please guide me

15 April 2024 8,620 0 View

If G1 has 40 DNA, what is number of DNA at end of Meiosis I ?

Options are a)40 b)80 c)20 d)60 Explain the answer.

09 April 2024 163 1 View

How to make a chamber airfroof for study of GHG ?

Want to study GHG emission from small ruminants in small chamber (low cost), How to make chambers airproof, if anyone having experience kindly share your experience with photo please. Thanking...

09 February 2024 5,288 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Method of collection of samples of microorganisms from environmets to labortary?

can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...

17 July 2024 3,687 11 View

Assistance with high taxonomic level identification of intertidal microalgae in SEM Images?

I am currently conducting research on benthic microalgae in the intertidal zone along the central California coast, specifically focusing on grazing by marine snails. As part of this study, I have...

08 July 2024 3,342 5 View

How can I improve purity of DNA extracted from cyanobacteria using Dynabeads?

I am trying to extract DNA from the cyanobacteria Microcystis aeruginosa using the Dynabeads DNA DIRECT Universal kit for the purpose of on-site detection. Yield is acceptable at ~100-500 ng/uL,...

01 July 2024 10,003 1 View

Which statistical test would be the best?

For my practical project at Uni, I am researching blue-green algae in a freshwater lake. I have three locations around the lake and am testing the water for phosphates, pH, and temperature. I am...

30 June 2024 4,264 3 View

How to perform the anaysis of taxonomic on raw reads from the mixed sample?

Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...

09 June 2024 6,537 3 View

Do algae have "vernalization"?

A few days ago, I saw a paper discussing the low temperature "vernalization" of microalgae (cyanobacteria). But in the paper, only low temperatures induced the growth effect of cyanobacteria was...

07 May 2024 2,908 0 View

How to correctly design and analyze microsatellite data for eukaryotes in Beast2 (BEASTvntr module)?

Dear Colleagues, We want to use microsatellite data to analyze the historical dynamics of mammalian populations and, in particular, to test the method of coalescent Bayesian scaling. We used...

17 April 2024 6,968 0 View

Why my phb polymer film stick to the beaker after chloroform extraction?

i have extracted cyanobacteria PHB and after some days it was found that the polymer in the beaker became stick and it was difficult to get it from beaker. I want to know the possible reason of...

09 April 2024 5,429 0 View

Need help with improving the 16S rRNA sequence quality with trimmomatic?

Hi all, I have just started to learn about bioinformatics and I need help with it. I have enriched some microbes from wastewater anaerobic sludge and sent them for 16S rRNA sequencing. Based...

29 March 2024 5,278 4 View