For finding a mutated cell line in ERRFI (MIG6), I use CCLE and COSMIC databases as well.
Both of them show that MIA PaCA2 has a Nonsense mutation in MIG6 and the protein length is 323 instead of 462 (wild type), but surprisingly after running the western blot for MIG6, I've found that the molecular weight for this guy is same with wild type cell lines in MIG-6 (for example Panc1).
Yes both resources show that there is this truncating mutation, but both indicate that it is heterozygous, so you still have a full length copy of this protein expressed in this cell line. And you may not see the 2nd LMW band if the antibody you are using has part or all of its recognition epitopes on the missing region.
Best
Amos
Hi Amos,
Thank you so much for the smart point that is mentioned.
But I can't figure out in which column I should recognize homozygous mutations from heterozygous?
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