We are new to the estradiol field and trying to measure levels in female rat serum. The kit I currently have (Arbor Assays Serum EIA Estradiol kit ) only measures unbound estradiol.
Will the unbound portion of estradiol fluctuate/cycle along with the total amount in circulation?
Which would we report in a paper? The unbound fraction only?
It is unclear if the "normal" levels of estradiol reported in papers are bound/unbound or both.
We could also measure the levels in urine if this provides a more reliable measure.
There appears to be a lot of speculation about the reliability of estradiol EIA measurements, but we do not have easy access to LC-MS or the funds to support that. We are looking for some guidance about how to proceed.
Thank you
Hello!
Usually, in the kit's instructions are indicated what form of hormone you'll determine Free or Total. If your kit are determined unbound hormone - you will see the level of free estradiol. In papers are specified what form of hormone were studied. The ratio free/total estradiol could be different and depends from many factors. It is important to choose the correct sampling time depending on the rat's cycle and time of day.
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