What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Effect of the an extreme weather event on milk yield across 100 farms. Do you think the below models reflect the research question?

I have a panel data of monthly milk yield as a dependent variable and extreme weather ( in terms of percentile-based days) and other independent variables on the other side of the equation. The...

04 October 2020 8,960 1 View

how to design my current controller with PI controller?

hallo, i want to design my current controller in my project 'Grid controlled converter". Iam not able to understand how to design my PI controller according to my controller. i also need to prove...

23 June 2019 7,810 3 View

Hallo guys, can anyone let me know how to calculate load flow conditions from abc to dq transformations for transmission lines?

i have checked in many documentations but i always find the same thing which transforms from abc to dq in a matrix form but if i want to do it in transmission lines where the generators, feeders,...

13 June 2019 6,418 3 View

How to calculate the stability constant values from cyclic voltametry data ?

In a system, if there is a formation of ML, ML2, ML3 and so on, can be the cyclic voltammetry technique would be helpful in determining the stability constants and other thermodynamic parameters...

01 February 2016 8,118 2 View

What is the difference between ESI and EI mass. How they are used for identifying structure?

Please suggest me answer. Thank U

13 October 2011 7,906 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Does sds interfere with immunization?

I want to extract a protein from polyacrylamide gel to be used for immunization. I am wondering if I need to run PAGE since sds might interfere with the immunization process. Thank you

10 February 2021 8,568 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View